Ruilu FENG

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Ruilu FENG

Ph.D. Candidate, YC Lab
Hong Kong University of Science and Technology
Hong Kong, China
Email: rfengaa @ connect.ust.hk

Short Bio

Ruilu FENG is a Ph.D. candidate at Drug delivery and biomaterials Laboratory in the Hong Kong University of Science and Technology, advised by Prof. Ying CHAU. She got the B.S. of Material Physics from the School of Chemical and Material Science, University of Science and Technology of China in 2017. She is interested in nucliec acid delivery (mRNA/DNA) , self-assembled peptide , and new biomaterial synthesis.

Publications

[11] mRNA Delivery and Storage by Co-Assembling Nanostructures with Designer Oligopeptides
Ruilu Feng, Atta Cheuk Yan Chang, Rong Ni, Jacky Cheuk Yin Li, and Ying Chau*
2022 Submitted
[10] Modification of Oligopeptide Assemblies Enhanced the Endosomal Escaping and Transfection of Viral Mimics
Ruilu Feng, Rong Ni, and Ying Chau*
2022 Submitted
[9] Comprehensive Optimization of Peptide-DNA Co-Assemblies for Gene Deliverys
Rong Ni, Ruilu Feng, and Ying Chau*
2022 Submitted
[8] A Peptide and the Selection Method Thereof.
Ying Chau, Rong Ni, Ruilu Feng, inventors; Yu Yu, Zhexun Sun, Pleryon Therapeutics Ltd., assignee.
Patent PCT/CN2021/138963. 17 December 2021.
[7] Altered Peptide Self-Assembly and Co-Assembly with DNA by Modification of Aromatic Residues
Ruilu Feng, Rong Ni*, and Ying Chau*
ChemMedChem, 2021, 16(23): 3559-3564.
[Paper]
[6] Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers
Rong Ni, Ruilu Feng, and Ying Chau*
Life, 2019, 9(3): 59.
[Paper]
[5] Rational Modification of Diphenylalanine Analog to Regulate the Morphology and Function of Peptide-DNA Co-assembled Viral Mimics
Ruilu Feng, Rong Ni*, and Ying Chau*
The 46th Controlled Release Society Annual Meeting & Exposition, CRS 2019, Valencia, Spain, 20-24 July 2019
[Poster]
[4] A facile methodology using quantum dot multiplex labels for tracking co-transfection.
Jessica A Kretzmann, Ruilu Feng, Alaa M Munshi, Diwei Ho, Anna M Ranieri, Massimiliano Massi, Martin Saunders, Marck Norret, K Swaminathan Iyer*, and Cameron W Evans*
RSC advances, 2019, 9(35): 20053-20057.
[Paper]
[3] Novel Metal Polyphenol Framework for MR Imaging-Guided Photothermal Therapy
Gaozheng Zhao, Huihui Wu, Ruilu Feng, Dongdong Wang, Pengping Xu, Peng Jiang, Kang Yang, Haibao Wang*, Zhen Guo*, and Qianwang Chen*
ACS applied materials & interfaces, 2018, 10(4): 3295-3304.
[Paper]
[2] Bimetallic Zeolitic Imidazolate Framework as an Intrinsic Two-Photon Fluorescence and pH-Responsive MR Imaging Agent
Gaozheng Zhao, Huihui Wu, Ruilu Feng, Dongdong Wang, Pengping Xu, Haibao Wang*, Zhen Guo*, and Qianwang Chen*
ACS Omega, 2018 Aug 23;3(8):9790-9797.
[Paper]
[1] Rapid Adsorption Synthesis Enables Interface Engineering of PdMnCo Alloy/Pyridinic Nitrogen-Doped Carbon as Highly Efficient Electrocatalysts for Hydrogen Evolution Reaction
Rui-Rui Zhang, Zhong-Ti Sun, Ruilu Feng, Zhiyu Lin, Haizhen Liu, MengsiLi, Yang Yang, Ruohong Shi, Wenhua Zhang, and Qianwang Chen*
ACS Appl. Mater. Interfaces, 2017, 9, 44, 38419–38427.
[Paper]

Research Experience

Drug delivery and biomaterial design Feb.2018 - Now
Advised by Prof. Ying CHAU @HKUST Hong Kong S.A.R.
Design peptide-based materials for mRNA/DNA delivery
  • Design of peptide-based material for mRNA delivery. By altering the peptide sequences and N/P ratio to mRNA, the new peptide (we name it pepMAX) achieved high mRNA transfection efficiency in various cell lines. One-week storage of lyophilized peptide-mRNA co-assemblies under room temperature showed no efficiency decline after reconstitution, which indicates this mRNA delivery technology would be applicable for room temperature transportation and storage. (12/2020-present)
  • Surface modification on the peptide self-assemblies or peptide-DNA co-assemblies for better endosomal escaping. Fusogenic peptide were added into the delivery system via click reaction, and the modified peptide-DNA co-assemblies achieved enhanced endosomal escaping and transfection efficiency. (08/2019-12/2020)
  • Characterizing the peptide self-assemblies and peptide-DNA co-assemblies morphologies and secondary structures, (including AFM, TEM, FTIR, CD scanning, 2D NMR). A molecular model was proposed to understand the peptide packing and interactions strength within the structures. (02/2018-04/2019)
    • Synthesis of rationally designed peptides for enhanced imaging ability in Computed Tomography (CT) Sep.2017 - Jan.2018 (Research assistant)
    Advised by Prof. Gaolin Liang @USTC Hefei, China
  • Rationally designed a peptide with alkaline phosphatase (ALP) catalysis ability, which self-assembled into nanofibrils for enhanced CT imaging for tumours
    • Fabrication of multi-coloured MRI contrast agents effective in low-intensity magnetic fields using the top down method Mar.2017 - Jun.2017 (Final year project)
    Advised by Prof. Julie Karel @Monash Uni Melbourn, Australia
  • Fabricated the MRI contrast agent with FIB-SEM and determined reasonable etching parameters for the designed the nanoparticles’ structure
    • Use of quantum dots (QDs) labels in tracking co-transfection with a dendronized polymer system Jul.2016 - Sep.2016 (Summer research)
    Advised by Prof. Killugudi Swaminatha Iyer @UWA Perth, Australia
  • Synthesized QDs under Schlenk condition and adjusted its ratio with PAMAM polymers/DNA to obtain ideal size and ζ potential for transfection and confocal tracking
    • Ellagic acid based multi-functional nanoparticles for cancer therapy Sep.2015 - Jul.2017
    Advised by Prof. Qianwang Chen @USTC Hefei, China
  • Synthesized ferric-ion-ellagic-acid nanoparticles (a MOFs nanoparticles) and characterized their morphologies. Confirmed particles’ H2O2, pH responsiveness, MRI contrast agent’s effect and photo-thermal ability

    • Skills

    Experimental Skills Cell culture, Transfection, MTT, Working with Schlenk condition, Synthesizing nanomaterials: solvothermal method, precipitation method, pyrolysis method. Synthesizing peptide
    Proficient Equipment TEM, AFM, FIB-SEM, Live cell confocal microscope, Fluorescent microscope, Plate reader, Flow cytometer, HPLC, UPLC, Peptide synthesizer, DLS, FTIR